Formulation and Characterization of Nanosuspension of Ethanolic Extracts of Malaxis Acuminata

Abstract

A nanosuspension is a submicron colloidal dispersion of drug particles which are stabilized by surfactants. The particle size distribution of the solid particles in nanosuspensions is usually less than one micron with an average particle size ranging between 200 and 300 nm. The present study was designed to formulate and characterized the nanosuspensions of Malaxis Acuminata plant extract. The nanosuspension of plants extract will be formulated using nano precipitation technique followed by its lyophilization using mannitol as a cryoprotectant. The nanosuspension was evaluated for their particle size, zeta potential, SEM and TEM. The particle size of formulations were in a range of 200-300 nm and PDI is 0.247 . The formulation was physically and chemically stable when stored at the 4-8°C and 40 ± 2°C at 75 ± 5 % RH for a period of one month. The nature of the nanoparticles synthesized from bulbs extract was analyzed by X-ray diffraction analysis an intensive peaks at 20.3022 and 28.5995 degrees of 2-theta (deg) on the XRD spectrum. Morphology study of drug particles in the Malaxis Acuminata nano suspension shows that it exhibit a spherical shape within the a size range. These particles are discrete, uniform in size, and show no indications of agglomeration. Top of FormIt can be concluded that Nanosuspension of Ethanolic Extracts of Malaxis Acuminata is stable.