Phytochemical Screening And Estimation Of Total Phenolics And Total Flavonoid Content Of Lagenaria Siceraria Fruit

Abstract

Lagenaria siceraria (Molina) Standley (Family: Cucurbitaceae) is commonly known as Bottle gourd, an excellent fruit in the nature having a composition of all the essential constituents that are required for normal and good health of humans. The aim of present study was to investigate the phyto-constituents present within the various extract of Lagenaria siceraria fruit and to estimate the total phenolic and total flavonoid contents. The amount of total phenols, were analyzed using a spectrophotometric technique, based on Folin-ciocalteau reagent. Gallic acid was used as standard compound and the total phenols were expressed as mg/g gallic acid equivalents (Standard curve equation: y = 0.009x + 0.0092 R² = 0.9982).The Rutin was used as standard compound and the total flavonoids were expressed as mg/g rutin equivalents (Standard curve equation:y = 0.0061x + 0.0085R² = 0.9986.