Pharmacological Screening Of Polyherbal Formulation For Hepatoprotective Effect Against Anti Tuberculosis Drugs Induced Hepatotoxicity On Albino Rats

Abstract

The present study was designed to evaluate hepatoprotective activity of poly herbal formulation(PHF)(Andrographis paniculata,Boerhaavia diffusa, Nigella sativa and Punica granatum)on Wistar albino rats using Anti Tuberculosis drugs  induced hepatic damaged experimental animals. Isoniazid and Rifampicin-induced was administered a 100 and 50 mg/kg bw dose to inducehepatotoxicity, PHF 200 and 300 mg/kg, p.o, and liv-52 syrup 5ml/kg, p.o.were administered once daily for 28 days. The degree of hepatoprotection was measured liver function serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), Serum alkaline phosphate and Total protein. Oxidative enzymes MDA,GSH,SOD and CATand histopathology of  liver.  Results obtained PHF significantly P decreases the   SGOT, SGPT ALP.  The histopathological parameters of liver architecture like architecture of hepatic lobules, swelling of liver cell, fatty changes, focal necrosis, inflammatory cell infiltration around portal areas, kupffer cell hyperplasia etc.The result of this study strongly indicated that the PHF has got a hepatoprotective action against Isoniazid and Rifampicin induced hepatic damage in experimental animals.