Supplementation With Dietary Lacto-Sacc in Earthen Pond Water Reduced Nitrofuran Derivatives in Mud Crab (Scylla Olivacea)

Abstract

Antibiotics, particularly nitrofuran derivatives, in aquaculture have raised major health concerns because to their carcinogenic and mutagenic properties. This study looks at how lacto-sacc, a probiotic supplement, decreases nitrofuran derivative residues in mud crabs (Scylla olivacea). Gravid female mud crabs were divided into three groups: control (0% lacto-sacc), 1% lacto-sacc, and 1.5% lacto-sacc. They were housed in breeding boxes inside earthen mangrove broodstock cages designed to simulate natural settings. The crabs were fed lacto sacc supplemented diets for 84 days before being tested for nitrofuran metabolites (SEM, AHD, AMOZ, and AOZ) by liquid chromatography-tandem mass spectrometry.  The results revealed that lacto Sacc-treated groups had considerably lower levels of nitrofuran metabolites than the control group. After 84 days, SEM levels in the 1.5% lacto-sacc group (T3) dropped to 0.49±0.03 ppm, AHD to 0.027±0.001 ppm, AMOZ to 0.008±0.012 ppm, and AOZ was absent. The study exhibited that lacto-sacc supplementation significantly reduced harmful nitrofuran compounds in mud crabs, promoting safer aquaculture operations.