Phytoconstituent Analysis Of Methanolic Extract Of Gracilaria Fergusonii Collected From Hare Island, Thoothukudi District, Tamil Nadu, India.

Abstract

The main objective of this present study was to investigate the phytoconstituent present in methanol extract of Gracilaria fergusonii collected from Hare Island, Thoothukudi District, Tamil Nadu, India. The phytochemical analysis of methanol extract was estimated using the standard procedure for UV-Vis spectroscopic, FT-IR and HPLC. The crude extracts of the selected Gracilaria fergusonii J.Ag. species was scanned in a wavelength ranging from 200-900nm. Seven functional groups were identified using FTIR spectrophotometer in the range of 4000 to 400cm^-1. The qualitative HPLC fingerprint profile of methanol extract of Gracilaria fergusonii J.Ag. was chosen at a wavelength of 254nm due to sharpness of the peaks and proper baseline. There are fifteen compounds were separated from Gracilaria fergusonii J.Ag. The profile observed five prominent peaks at the retention time of 1.483min, 1.570min, 1.743min, 1.850min, 2.017min, followed by ten moderate peaks were predicted at the retention time of 1.273min, 2.247min, 3.520min, 3.850min, 4.220min, 4.517min, 4.703min, 5.107min, 7.037min and 12.230min.