Correlation Between Tumor Necrosis Factor-Α (TNF-Α) And Bacteria Endotoxin An In Vitro Study

Abstract

Gram-negative bacterial lipopolysaccharide (LPS) which has been demonstrated to induce inflammatory reactions in vitro as well as in vivo, including lethal shock. A great number of different cells have been documented to be reactive to LPS, e.g. monocytes/macrophages, vascular cells, polymorph nuclear cells, and even B lymphocytes. We have now established that T lymphocytes could also contribute to an inflammatory reaction to LPS. LPS is a potent inducer of human T-lymphocyte proliferation and cytokine production, macrophages by interacting with Toll-like receptor 4 (TLR4) and triggers the production of various pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and clearance of infectious organisms. Systemic inflammation is implicated in the pathophysiology of many diseases, including chronic inflammatory diseases, infections, sepsis, atherosclerosis, and obesity. This experimental study was performed to find out the effect of healthy person on peripheral blood mononuclear cells(PBMCs) namely T-cells and natural killer cells(NK cells) function through challenging  their ability  for cytokines production in a comparative study by using of standardized inoculum of Bacterial endotoxins